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The reduction level glutathione (GSH) in tomato leaves was estimated by homogenizing fresh sample tissue in 5% (w/v) of sulpho-salicylic acid (Smith, 1985)

Specifically, it is known that among the different forms of vitamin B3 (NAD precursor), transport of Na is mediated by membrane carrier systems potentially including either a pH-dependent anion antiporter or a proton cotransporter ( eNAD can bind different subtypes of purinergic P2 receptors, including P2Y11, leading to the opening of L-type Ca 2+ channels and activation of a cAMP/cADPR/[Ca 2+ ]i signaling cascade, ultimately causing increased proliferation and migration ( 2+ internalization, opening a non-selective, large membrane pore, causing cell death ( The very low levels of eNAD are due to its rapid metabolism/degradation by NAD-catabolic enzymes present on the surface of the cell ( eNAD is degraded by three main classes of specific ectoenzymes: CD38 and CD157 (Figure 1)

GSH concentration in sample can be determined by subtracting glutathione disulfide concentration from total glutathione concentration

Giang Son Arrighini: Project administration, Methodology, Data curation, Conceptualization

Sorolla MA, Reverter-Branchat G, Tamarit J, Ferrer I, Ros J, Cabiscol E

Vernon SD, et al